Antigen

Antigen Class

TFs and others

Antigen

METTL14

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

cell line

K562

cell type

Human immortalized myelogenous leukemia cell line

genotype

WT

chip antibody

Rabbit polyclonal anti-METTL14; Sigma-Aldrich; Cat#HPA038002

geo_loc_name

missing

collection_date

missing

Sequenced DNA Library

library_name

GSM7147759

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde. ChIP was preformed with the iDeal ChIP-seq kit for Histone marks (Diagenode) or the the iDeal ChIP-seq kit for Transcription Factors (Diagenode) Library preparation was performed with KAPA HyperPrep Kit for NGS DNA Library Prep (Roche) according to the manufacturer's protocols.

Sequencing Platform

instrument_model

NextSeq 550

hg38

Number of total reads

22206633

Reads aligned (%)

82.4

Duplicates removed (%)

18.4

Number of peaks

11809 (qval < 1E-05)

hg19

Number of total reads

22206633

Reads aligned (%)

81.6

Duplicates removed (%)

19.7

Number of peaks

11711 (qval < 1E-05)