Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
METTL14

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
cell line
K562
cell type
Human immortalized myelogenous leukemia cell line
genotype
WT
chip antibody
Rabbit polyclonal anti-METTL14; Sigma-Aldrich; Cat#HPA038002
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM7147759
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde. ChIP was preformed with the iDeal ChIP-seq kit for Histone marks (Diagenode) or the the iDeal ChIP-seq kit for Transcription Factors (Diagenode) Library preparation was performed with KAPA HyperPrep Kit for NGS DNA Library Prep (Roche) according to the manufacturer's protocols.

Sequencing Platform

instrument_model
NextSeq 550

hg38

Number of total reads
22206633
Reads aligned (%)
82.4
Duplicates removed (%)
18.4
Number of peaks
11809 (qval < 1E-05)

hg19

Number of total reads
22206633
Reads aligned (%)
81.6
Duplicates removed (%)
19.7
Number of peaks
11711 (qval < 1E-05)

Base call quality data from DBCLS SRA